Why can I not detect other targets than GAPDH?

Hello, we're trying to detect Keratin 14 and 15 in Keratinocytes as well as FN-1 and CD90/THY1 in Fibroblasts from human skin. As reference gene GAPDH and Vinculin are used. In our first...

07 January 2021 6,652 3 View

Does anyone know instruments to measure "mental health literacy" in teachers?

Hi there, I am looking for questionnaires measuring mental health literacy in teachers and educators. does anyone know any studies in this area?

16 September 2020 1,682 4 View

Which solution do you use for low level actinide decontamination of laboratory surfaces like fume cupboards, work tops or floors?

Efficient decontamination of actinides like americium, uranium, neptunium and plutonium from laboratory surfaces. The decontamination should be easy in the sense that it could be performed on a...

03 June 2020 8,707 5 View

How to do a non-linear regression analysis with multiple variables and a strong confounding variable?

Hi, I did outgrowth assays on spiral ganglion neurons from the inner ear with each supplemented by different concentrations of two neurotrophins. For each explant I did an image analysis and...

29 March 2020 8,703 7 View

Would somebody have suggestions for bioinformatic tools that predict changes in enzymatic (e.g. kinase) function based on changes in AA sequence?

Prediction of enzymatic activity based on common genetic variation.

19 February 2020 6,582 2 View

What are minimum and maximum values for ratios in Geopolmers (Si:Al, Na2O:H20, NaO:SiO)?

I am trying to perform a screening DoE to further study influence of a range of factors for example Metakaolin content on various performances like compression strength and to further optimise my...

23 January 2020 5,020 3 View

How to compare diagnostic efficacy of several methods in a within-subjects design?

Hi, I conducted a study on faking in personality tests, where the participants received two instructions (respond honestly vs. fake good) in a within-subjects design (oder was counter-balanced...

09 January 2020 5,182 3 View

Why do I get a different results (load-displacement-curve) when applying different loads in ABAQUS/Explicit?

I'm currently modelling a steel-concrete composite construction with headed studs in ABAQUS/Explicit and want to analyse the load-displacement behaviour of the headed stud. In the first case I...

18 December 2019 3,070 4 View

What amount of data is necessary to extract meaningful/significant Sequential patterns / rules in a sequential database?

10 March 2019 5,559 3 View

Mcfarland or OD600?

Which is more reliable to give as an indicator of bacterial cell culture concentration, for example your typical "overnight starter culture": Mcfarland standards, or OD600? which do you prefer...

17 January 2019 3,506 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View