What is the best way to freeze-dry (lyophilise) the extracted DNA?
we need high quality for NGS
Preservation of nucleic acids by freeze-drying for next generation sequencing analyses of soil microbial communities
Check https://www.sciencedirect.com/science/article/pii/S1164556313000332
My question is about the extracted DNA not tissue or other sample
The core was sliced under nitrogen-enriched atmosphere using sectioning material previously sterilized with bleach and cleaned for trace metal sampling with trace metal grade nitric acid. The core was sectioned every 1 cm from depths 0 to 20 cm and subsequently every 2 cm from depths 20 to 46 cm. Half of each sediment slice was immediately stored at −20 C and freeze-dried for a period of 72 h under a vacuum of 10−5 atm with a Pilot PCCPLS15 freeze dryer (Cryotec) and then homogenized. These lyophilized sediments were further used for Hg content determination as well as DNA extraction. The other half (subsequently called “frozen” sediments) was immediately rinsed with a sterile buffer (SB) composed of 10 mM EDTA, 50 mM TrisHCl.
I got from this paper.
https://peerj.com/articles/11075.pdf
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