for Identification of Bifidobacterium stains we need pure genomic DNA for 16s rRNA PCR and sequencing
Bifidobactrium is one of gram positive bacteria. you can use DNA extraction kit(thermo fisher) for good and pure yield of bacteria.
Dear Shahrzad Ahangarzadeh thank you for asking this interesting technical question. In this context please have a look at the following useful articles which might help you in your analysis:
Probiotic DNA Extraction of Bifidobacterium lactis and Lactobacillus fermentum via High Osmotic Dual- Bead Beating Technique
https://herbalifenutritioninstitute.com/wp-content/uploads/2018/08/Probiotic-DAN-Extraction-of-Bifidobacterium-Lactis-and-Lactobacillus-Fermentum-Via-High-Osmotic-Dual-Bead-Beating-Technique.pdf
(see attached dpf file)
An improving DNA isolation method for identification of anaerobic bacteria in human colostrum and faeces samples
https://academicjournals.org/journal/JMGG/article-full-text-pdf/E3982538574
and
Detection and quantification of probiotic bacteria using optimized DNA extraction, traditional and real-time PCR methods in complex microbial communities
(also attached)
Good luck with your research and best wishes, Frank Edelmann
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