17 March 2022 4 6K Report

I am working on P. aeruginosa Biofilm assays as a part of my research. Biofilm formation was carried out using Microtiter plate Biofilm Formation Assay.

  • Can someone suggest a way to find MIC of the biofilms using the normal 96 well plates? I tried to get the results using the microdilution method, but it gave me smaller MIC values for the Biofilms compared to the Planktonic Cells.
  • Is 620 nm wavelength too high to quantify the biofilm in 30% acetic acid? Because the plate reader in our lab provides readings only at 492nm and 620nm.

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