What is the best way to Estimate the DNA damage?

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Sarhan Rashid Sarhan

thank you jiang kunpeng@

Alex Lyakhovich

it is a transit process coupled with DNA repair. kinetic depends on the system you work with. as such, any readout for your DNA damage (e.g. amount of gamma H2AX by Western, or accumulation of pyrimidine dimers by IF or looking for telomere attrition by qFISH) will be inaccurate until you focus on specific type of DNA damage (ssb? dsb? oxydation? alkylation?) and consider specific type of DNA repair.

Arideep Mukherjee

Suggested protocols for assessing DNA Damage

https://www.nature.com/articles/nprot.2006.5

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5452911/

Article Evaluation of DNA damage using single-cell gel electrophores...