I am trying to immunoprecipitate LKB1 which is a protein with a molecular weight very close to IgG. I want to cross-link my antibody to the beads to help solve this problem.
Is this the best way to do it (crosslinking the antibody to the beads) or is there a more efficient way?
The simplest way to do it is to buy NHS-activated magnetic beads for conjugation to lysines on your Ab. The downside is that the beads are not particular stable during storage unless free of moisture, but once conjugated the link to the antibody is irreversible. If you are not used to doing reactions, this is your best option I think, and there is a high chance of success
There are plenty of other chemistries too, and nearly all of them also create a bond between lysines and the bead. So the specifics of the linker between the bead and antibody may change, but otherwise the Ab is coupled through the same lysines. Don't bother with complex site-directed protocols; you resort to these approaches only if the simple methods don't work.
This protocol is great too
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/immunoprecipitation/dynabeads-immunoprecipitation-crosslinking-protocol.html
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