SPSS: A scale with multiple items (7-point Likert) across 4 test conditions. How can I correctly summarize it?

Hello, I am currently writing my master thesis where I have a 2x2 factorial design with one downstream variable (2 independent and 2 dependent variables). I need to conduct a multivariate...

11 February 2021 6,014 4 View

Hi, help please, how to cancel the authorship of the article Civilizational Phenomenon of Narcissism: Vectors of Objectification in a Paradigm...?

Hi, help please, how to cancel the authorship of the article Civilizational Phenomenon of Narcissism: Vectors of Objectification in a Paradigm of Psychoanalysis ?

15 December 2019 9,043 3 View

Where I can order fungal ITS1 and ITS4 primers?

I would greatly appreciate for help in the suggestion of company for ITS1 and IT4 primers purchasing in Europe. Thank you.

12 September 2017 469 3 View

Could the gene silencing be avoided when it is expressed from the episomal plasmid?

In the case of yeast transformants

14 March 2016 9,434 1 View

Does anyone know some kits for express diagnostics basic soil characteristics?

Dear colleagues, I need to estimate the basic soil characteristics - total carbon, nitrogen, potassium content. I also need the content of Fe and halogens (F, Cl, maybe I). The point is that I can...

12 February 2015 4,874 9 View

Has anyone used Mesquite for combining molecular and morphological data? Do you know a algorithm for doing that?

Phylogenetic analysis (combining molecular and morphological data).

10 August 2014 4,406 7 View

Could you please suggest a technique of a plant root preparation for XRF confocal imaging?

Zou Finrock and Kelly Summers have recommended me to contact you. We are using 8-day old Arabidopsis plants to see of the effects of toxic metals, in the root tips, in particular. They are really...

01 January 1970 5,600 3 View

What do you think about the status of ownership in the charing economy?

We understand that in sharing economy, access to usage will be important. How will the attitude to ownership and disposal of property change? Do you want be the owners?

01 January 1970 546 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View