Dear reaserchers,
I study biohydrogen production via dark fermentation by activated sludge microorganisms. We conduct lab scale batch-experiment in AMTPS Bioprocess Controll (working volume of single reactor is 400 ml). I wonder what is the best way to collect the biogas samples from our experiments. So far, we have taken samples by connecting the batch reactor with a tedlar bag (volume 600 ml) with a hose. Unfortunetely this way is not good enought for avoid air contamination of the samples and rapid hydrogen dyffusion.
I found an interesting paper that eliminates the need for GC and provides continuous real time monitoring of Hydrogen and Methane in small bioreactors.
https://www.ecorfan.org/spain/researchjournals/Investigacion_y_Desarrollo/vol5num16/Journal_of_Research_and_Development_V5_N16_1.pdf
I would recommend foil bags with screw valve fittings for collecting biogas over standard tedlar bags. Foil bags generally have better stability for low molecular weight compounds and are less prone to oxygen diffusion. I also think that bags with a built in screw valve are less prone to leakage as well as being easier to connect to fittings.
Though you can easily test if the bag is your source of contamination by filling it with a known gas, leaving it for a representative time and then analaysing the gas.
Thank you so much for all your advices and recommendations. I hope that I will optimize my biogaz sampling.
- Badges
- Science topic