I've been using Hoecsht staining and Crystal violet staining to normalize my experiments but I have found that Hoechst cannot detect less than a 1000 cells. Is there a good assay or way to find the actual cell count?
Según el Ministerio de Tecnología e Innovación Colombiano, los entonos estrategicos en los que deben trabajar los gobiernos para adoptar una posición eficiente frente a la Inteligencia artificial,...
23 June 2024 9,844 1 View
I am considering undertaking a quant + qual research by providing teachers with a questionnaire to measure their feelings and practice with regards to a type of music. I was thinking of...
14 March 2024 5,757 11 View
Cómo el racismo que está integrado en las estructuras sociales afecta a las comunidades que ya están en riesgo de exclusión, como las comunidades racializadas o marginadas, y cómo esto hace que...
10 March 2024 1,491 3 View
Todo el mundo alega que si ocurriese una injusticia lo correcto sería contarlo pero, cómo se debería dar voz de forma adecuada en este caso, sin poner en riesgo a las víctimas?
10 March 2024 5,202 1 View
Qué método sería el más adecuado para investigar este colectivo, de manera ética y respetuosa, para obtener información relevante sin violar sus derechos.
10 March 2024 612 1 View
Normalmente se suelen utilizar esos dos términos coloquialmente a la hora de hablar sobre el tema, y me gustaría saber las diferencias para llevar el tema con propiedad y que más personas sean...
10 March 2024 2,567 1 View
I need to evaluate abdominal adipose tissue hypertrophy in a murine model with metabolic syndrome, with and without treatment and I need to review which is the best objective to measure adipocyte...
09 January 2024 7,149 0 View
Dear colleagues, I am trying to plot a protein-protein interaction network in Cytoscape. My idea is to place the node representing the bait protein on one side of the figure, and on the other...
18 December 2023 9,182 2 View
The liver sections look fragmented, how could the histological technique be improved to better observe this tissue under the microscope?
06 December 2023 7,388 3 View
I am analyzing the standardized mediating indirect effect in AMOS.
19 November 2023 3,250 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View