Alfredo Reyes Oliveras

6 Questions 1 Answers 0 Followers

Questions related from Alfredo Reyes Oliveras

Can I do an overnight enzymatic reaction after fixing cells with 4% PFA at 37 C?

I want to detect glycans with lectins by flow cytometry. One of the glycan I’m interested I need to do an enzymatic reaction overnight at 37 C to expose the epitope. Can I leave the cells after...

29 July 2021 9,751 1 View

Why am I getting narrow lanes in some of my samples after electrophoresis?

Hi, I ran SDS-PAGE followed by a wet transfer into a PVDF membrane, but I did not get a good transfer. For cell lysis, I used RIPA buffer. I used Fluorescent Compatible Sample Buffer (4X) and...

18 February 2021 5,457 3 View

Can cell lines have different DNA content?

I am doing calibration curves using CyQUANT Direct Assay to 10 different pancreatic cancer cell lines. I've noticed that the fluorescence of some cell lines double at the same cell number. Is this...

04 February 2021 3,901 2 View

What is the best way or assay for cell count normalization?

I've been using Hoecsht staining and Crystal violet staining to normalize my experiments but I have found that Hoechst cannot detect less than a 1000 cells. Is there a good assay or way to find...

08 November 2020 2,169 0 View

When doing electrophoresis how the thickness of the gel affects the results?

I am doing an electrophoresis using a Tris-Acetate gel and the vendor offers a 1.0 mm and 1.5 mm thick gels. I just want some insight on the advantages. Thank you

08 October 2020 3,393 2 View

Can I incubate two secondary antibodies at the same time for western blot?

I want to know if I can use a goat-anti mouse IgM-HRP and a goat-anti mouse IgG-HRP to treat a western blot membrane at the same time. Thank you

27 September 2020 1,581 6 View