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Questions related from Alfredo Reyes Oliveras
I want to detect glycans with lectins by flow cytometry. One of the glycan I’m interested I need to do an enzymatic reaction overnight at 37 C to expose the epitope. Can I leave the cells after...
29 July 2021 9,806 1 View
Hi, I ran SDS-PAGE followed by a wet transfer into a PVDF membrane, but I did not get a good transfer. For cell lysis, I used RIPA buffer. I used Fluorescent Compatible Sample Buffer (4X) and...
18 February 2021 5,502 3 View
I am doing calibration curves using CyQUANT Direct Assay to 10 different pancreatic cancer cell lines. I've noticed that the fluorescence of some cell lines double at the same cell number. Is this...
04 February 2021 4,131 2 View
I've been using Hoecsht staining and Crystal violet staining to normalize my experiments but I have found that Hoechst cannot detect less than a 1000 cells. Is there a good assay or way to find...
08 November 2020 2,214 0 View
I am doing an electrophoresis using a Tris-Acetate gel and the vendor offers a 1.0 mm and 1.5 mm thick gels. I just want some insight on the advantages. Thank you
08 October 2020 3,451 2 View
I use CelltiterGlo to quantify ATP per cells. I made 3 ATP solutions at different concentrations (1000 nM, 100 nM, 10 nM and without ATP) to make a standard curve. Can I freeze them and re use...
08 October 2020 1,263 3 View
I want to know if I can use a goat-anti mouse IgM-HRP and a goat-anti mouse IgG-HRP to treat a western blot membrane at the same time. Thank you
27 September 2020 1,759 6 View
