Many serological tests apparently have insufficient diagnostic performance (sensitivity, specificity, predictive values). What research protocols are used to validate these tests? What is the gold standard used?
La politique de financement est en soit une politique publique. Comment les évaluer dans un travail de recherche scientifique ?
22 July 2024 6,495 2 View
Hi all, I am looking for some syntethised samples of mixed-layer clays to perform infrared reflectance spectroscopy measurements in the VNIR-SWIR-MWIR-LWIR regions. Best regards, Lionel Fonteneau
01 July 2024 3,875 2 View
Please help me to know how an elasticaly mounted circular cylinder for VIV model can be used in Civil Engineering.
12 December 2023 2,497 3 View
Co-expression of multiple proteins in E. coli requires the co-transformation of several plasmids. What is the best protocol for the preparation of ultra-competent E. coli BL21?
29 June 2023 495 3 View
Please, I want some article which talk about the RHA effect on geopolymer concrete properties.
11 June 2023 7,460 6 View
I was reading the T7 RNA polymerase protocol and noticed the following sentence regarding incubation time: "Incubate at 37°C for 1 hour. For shorter (< 300 nt) transcripts incubate at 37°C for...
17 June 2020 1,918 0 View
Extraction of viral RNA from cell supernatants or from plasma/serum is supposed to be possible using TRIzol or TRIsure, provided that glycogen is added. What is your own experience? I want to...
23 March 2020 7,881 7 View
Hello, Has anyone used the Roche HighPure viral RNA kit to extract RNA from cells? The idea for me is to use the same kit to extract RNA from supernatants of infected cells and from the cells...
23 March 2020 3,939 3 View
I am planning to run a glucose uptake experiment in mice (In vivo). I would like to inject H3-2DG and just measure the percent of H3 in different tissues. No clamp experiment, but I was thinkMing...
18 February 2020 9,669 3 View
Hi, we are going to purchase the AriaMx qPCR system from agilent. One of the reasons we choose this system is the possibility to add filters cartridge later to upgrade the system. Thus, we would...
24 February 2019 7,861 3 View
I am working on a project proposal (DFG project in Germany). I have the problem that I want to calculate how much funds I have to request for my test subjects (e.g., for qualitative interview of...
18 July 2024 1,690 0 View
I am working on SCE and micronucleus assay. I completed around 30 samples of SCE and MN but suddenly the results get stopped and its been above 1 year struggling with the cell culture experiment....
02 July 2024 7,649 3 View
I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...
02 July 2024 9,807 3 View
How Can I Determine Sample Sizes for Diagnostic Test Studies?
23 June 2024 3,612 5 View
How can I use EDSV fiber protein antigen for hemagglutination test?
05 June 2024 1,459 0 View
I am analyzing my data using Permutation Tests. I have the codes for a permutation test for the t-test, but I need codes for a permutation test for repeated measure ANOVA and a permutation test...
01 June 2024 1,211 2 View
I am conducting a study on whether the visual outcomes differ before and after surgery in glaucoma patients depending on the type of intraocular lens (IOL) used. I am examining whether there are...
22 May 2024 1,459 2 View
I've used the basic formula : 95% confidence interval = sensitivity +/− 1.96 (SE sensitivity) Where SE sensitivity = square root [sensitivity – (1-sensitivity)]/n sensitivity) 95% confidence...
21 May 2024 2,227 1 View
Profenophos is not soluble in water ? but solule in acetone? By useing acetone how to prepare diffent concentration pesticide ? And to find LC 50 for Profenophos?
22 April 2024 5,121 3 View
This research question delves into the multifaceted impact of integrating the Sysmex DI-60 hematology analyzer with several advanced features—automated sample preparation, advanced flagging...
12 April 2024 1,708 1 View