Efficient TRIzol/TRIsure viral RNA extraction?

Extraction of viral RNA from cell supernatants or from plasma/serum is supposed to be possible using TRIzol or TRIsure, provided that glycogen is added. What is your own experience? I want to...

23 March 2020 7,767 7 View

Does someone have a protocol for glucose uptake experiments in vivo?

I am planning to run a glucose uptake experiment in mice (In vivo). I would like to inject H3-2DG and just measure the percent of H3 in different tissues. No clamp experiment, but I was thinkMing...

18 February 2020 9,531 3 View

How to separate plasmid and genomic DNA?

I have a total DNA mix containing plasmid DNA and genomic DNA. I would like to recover the genomic DNA fraction and not plasmids from this mix and I don't want to use cesium chloride. Can anyone...

29 January 2014 9,406 19 View

What are the methods to identify qualitatively, and quantitatively, the bacterial community of samples?

I do not wish to use the time-consuming cloning/sequencing methods or costly 454 GS platform. The Ion Torrent PGM technology seems to offer modest set-up and running costs. What are your experiences?

04 March 2013 3,506 1 View

Should a new medical device be evaluated on its own (such as a drug) or integrated with its end-user and its environment?

The diversity of medical devices, their increasing complexity, as well as the development of devices for personal use have increased the risk associated with misuse. The use made by "operators",...

01 January 1970 2,096 2 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Can anybody provide me sequences of SPF10 primers?

03 February 2021 1,053 2 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

Can anyone help for the content validity of my psychological test?

Good day! I am in the midst of completing my test development requirement under the Psychological Assessment subject. I was wondering if anyone can help me reach experts or psychology graduates...

14 January 2021 1,536 4 View

Which neuropsychological tests assess immediate and delay(24-48hrs) declarative memory/learning (verbal and visual) and procedural memory in children?

it can be either a neuropsychological assessmentfrom age 8 years old or a wel research paradigm

13 January 2021 2,308 2 View

Spatial Panel Diagnostic Test?

Hello Colleagues Trust you are all fine, Please I have been struggling for days on estimating some spatial diagnostic test on my model. The stata command I am using is spatdiag command. I am...

04 January 2021 3,919 1 View

Has anybody knows about Anti-COVID-19 antibody- anti-Viral COVID-19 Nucleocapsid (NP) Humanized Coronavirus?

In order to establish new Rapid Diagnostic Test, I need the mentioned Anti-COVID-19 antibody urgently. Has anybody can assist me to prepare it? Thanks in anticipated

31 December 2020 635 5 View

Calculate sample size for diagnostic tests?

What sample size formula should I use to compare the three area under the roc curve (AUC) obtained from three diagnostic methods from similar sample? MedCalc software gives us the sample...

20 December 2020 5,635 3 View

Hosking's Multivariate test?

Hi. I'm running a multivariate GARCH model. When I introduce un AR(1) in the mean equation, all the diagnostic tests on Standardized Residuals and squared residuals are perfect (Q-Statistics,...

18 December 2020 9,898 2 View