I have done ABTS assay for some of my plant extracts as per the following method -
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231 – 1237.
I have calculated the IC50 values of my extracts as well as some standard antioxidants including trolox. I found the IC50 values of my extracts in the range of 50-200 µg/ml. I found the IC50 value of trolox and ascorbic acid to be 14.78 and 17.06 µg/ml, respectively. I am feeling difficulty in expressing the antioxidant capacity in terms of TEAC. Please suggest me how to to it exactly and in what units they should be expressed?
Hello Nirbhay,
It is hard for a plant extract to say equivalent to a single compound like Trolox (Tx) or Ascorbic acid (AA). Usually, the main reason to use a standard drug or compound is to validate the model. Therefore, it is not necessary that you must have to mention the equivalent parameters of one sample. You can just mention the IC50 of your sample. But if you really want to mention then go for the IC50 values with 95% confidence level and mention ~µg/ml of extract is equivalent to ~µg/ml of Tx or AA.
Hello Nirbhay,
It is hard for a plant extract to say equivalent to a single compound like Trolox (Tx) or Ascorbic acid (AA). Usually, the main reason to use a standard drug or compound is to validate the model. Therefore, it is not necessary that you must have to mention the equivalent parameters of one sample. You can just mention the IC50 of your sample. But if you really want to mention then go for the IC50 values with 95% confidence level and mention ~µg/ml of extract is equivalent to ~µg/ml of Tx or AA.
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