What is the best tlc solvent system for extracting tanin, alkaloid and terpenoid from a methanol crude extract of a plant belonging to a Rubiaceae?
Dear Dr. Joy,
You will simply need to work out your own solvent systems by considering some factors. Try a combination of different ratio of solvents. Also consulting literature on the subject will also help. However, an excerpt from our book project by Banjo et al. indicates that:
1. Water is good for the extraction of anthocyanins, starches, tannins, saponins, terpenoids, polypeptides and lectin.
2. Ethanol is good for the extraction of tannins, polyphenols, polyacetylenes, flavonols, terpenoids, sterols and alkaloids.
3. Methanol for the extraction of anthocyanins, terpenoids, saponins, tannins, xanthoxylene, totarol, quassinoids, lactones, flavones, phenones, and polyphenols.
4. Chloroform is used for the extraction of terpenoids and flavonoids
5. Ether is used for the extraction of alkaloids, terpenoids, coumarins and fatty acids.
6. Acetone is used for the extraction of phenol and flavonols.
From the above, a combination of Methanol:Ether:Chloroform or Ethanol:Ether:Chloroform may be good.
Observe the trend occurring in the ubiquitous terpenoids also.
Consider other factors and work things out.
Thank you.
Kind regards,
Egbuna, C.
httts://egbunac.com
What is the best food? Who is the best author ever? There are no single answers. The same is true when it comes to TLC solvents. It is even worse when you try to find a single solvent system for 3 wildly and widely different compound classes. I have attached a few articles that might help. But rather than trying to find a single golden solvent, use 3 (or more) solvent systems to get the best results.
Also, note that the tannins (and other flavonoids) will oxidize and generate new compounds depending upon the age of the sample, how it was extracted and other exposure to oxygen. Sometimes antioxidants are added to prevent this but they cause their own problems.
To add to Dr. Olechno, you should not depend only on one solvent system and no single stationary phase should be used. Based on the properties of the material, you may need to switch from silica to basic alumina or neutral alumina. Sometime, you may need to add ammonium hydroxide, trimethylamine, and even acetic acid to your mobile phase.
Dear Dr. Joy,
You will simply need to work out your own solvent systems by considering some factors. Try a combination of different ratio of solvents. Also consulting literature on the subject will also help. However, an excerpt from our book project by Banjo et al. indicates that:
1. Water is good for the extraction of anthocyanins, starches, tannins, saponins, terpenoids, polypeptides and lectin.
2. Ethanol is good for the extraction of tannins, polyphenols, polyacetylenes, flavonols, terpenoids, sterols and alkaloids.
3. Methanol for the extraction of anthocyanins, terpenoids, saponins, tannins, xanthoxylene, totarol, quassinoids, lactones, flavones, phenones, and polyphenols.
4. Chloroform is used for the extraction of terpenoids and flavonoids
5. Ether is used for the extraction of alkaloids, terpenoids, coumarins and fatty acids.
6. Acetone is used for the extraction of phenol and flavonols.
From the above, a combination of Methanol:Ether:Chloroform or Ethanol:Ether:Chloroform may be good.
Observe the trend occurring in the ubiquitous terpenoids also.
Consider other factors and work things out.
Thank you.
Kind regards,
Egbuna, C.
httts://egbunac.com
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