What is the best time to study ERK1/2 and CREB phosphorylation in mice hippocampus?

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João Covita

In this article the authors do 30 min exposure for ERK and 60 for CREB to several NMDA concentrations.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615746/

Still, I'd say it may differ if you're working with slices instead of cultures. Hope it helps!

Eric Wang

For pcreb, I've done 90minutes with good results (in vivo), but like Joao said, it probably depends on location, slices/cultures/vivo, etc.