Qualitative Analysis Software Recommendations?

I am looking for expert recommendations on qualitative analysis software. I have been engaging in more mixed-method work recently and I need reliable qualitative analysis software. Any...

02 February 2021 7,984 7 View

How to tackle autofluorescence using the technique FISH in mouse frozen tissue?

Hi there, I am looking for some advice with FISH. This is my first time attempting this technique and I am not too sure where I could potentially be going astray. I have followed FISHTag RNA...

23 September 2020 3,849 1 View

What are the best emotional intelligence scales that contain subdimensions?

We are looking to do a study on leadership and we want to use a an Emotional Intelligence Scale that meets the following criteria 1. is used in published academic research, not just consulting...

14 July 2020 7,122 2 View

Stability of N-Benzyl substituted indazoles?

I am working with N-Benzyl substituted indazole-nucleus but I'm having many problem with the stability of the molecules. Does anyone know if the benzyl position is sensitive to something, such as...

29 June 2020 7,505 3 View

How to normalize a Raman spectrum of graphene oxide to calculate ID / IG?

I am normalizing with respect to the G band, in this way I obtain as Raman intensity for this band a value of 1.00 and for the D band a value of 1.42, in this way I calculate ID / IG = 1.42, I...

21 December 2019 6,923 3 View

CAMKII-alpha triple immunofluorescence help in Primate?

I'm having a heck of time! I'm trying to perform triple immunofluorescence for CAMKII (alpha; mouse; milipore 05-532; 1:5K; visualized with AlexaFluor 647), Fluorescein (Invitrogen, A11095, goat,...

29 October 2019 7,063 2 View

Phosphorylated and non-phosphorylated protein X in the same western via IR fluorescence secondary?

The size of protein X (Phosphorylated and non-phosphorylated) will be pretty much the same on a western. Let's say after primary antibodies incubation of the two separate proteins (Phosphorylated...

16 September 2019 5,583 5 View

Oil Red O staining protocol - best mount to use?

Hi, I am having trouble with my Oil Red O stain for my rat MSCs. What is the best aqueous mounting medium to use? I followed the protocol from IHCWorld...

03 April 2019 10,012 3 View

Statistical Method for Treatment Outcome Research?

Hello all, I am completing a paper on the change in impairment over the course of treatment. I am not only interested if seeing that there is change overall, but where the biggest change occurs...

24 February 2019 3,709 4 View

Quantification of osteoblast staining?

Hi, I am planning on seeding rat MSCs onto 6 well plates with coverslips attached on the bottom. I then want to use stains such as Alizarin Red and Von Kossa to see the level of osteogenic...

02 February 2019 7,584 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View