Kelly A N Messingham

2 Questions 2 Answers 0 Followers

Questions related from Kelly A N Messingham

What is the best tag for large scale (>100 ml) purification of a large protein expressed in mammalian cells with EDTA (no 6XHIS)?

We are re-cloning due to introduced mutations in our old line so any suggestions are welcome

11 January 2018 831 1 View

Selective degranulation inhibitor/trigger to differentiate the effects of mast cell and eosinophil degranulation?

We are trying to determine if human mast cells and eosinophils are modulating degranulation of the other cell type when triggered ex vivo. We are triggering degranulation by adding antigen...

14 May 2013 3,532 0 View