I want to inject B16F10 cells into C57BL/6 mice as a melanoma metastases model to study the speed of metastases in different organs. I need advice about the designing of the best timeline for studying metastases in organs.
Hi Maryam
I am also going to use the same method, when I checked the literature it seems that tumors must be palpable approximately 300-400mm3 in volume, and according to the ethical committee's guideline animal should be sacrificed when the tumor size is less than 1500mm3, which based on what I read in most of the publications it's around or less than 4 weeks after inoculation of 5*10e6 cells subcutaneously.
I recommend this article Article Age effects of distinct immune checkpoint blockade treatment...
if you check their graphs you will see the control group usually did not last more than 4 weeks.
The graft success rate is also another factor you should consider some people use matrigel to have more consistent results.
one problem we face frequently is the initial detection of the tumor. sometimes it can be misleading especially the first few days when there might be an inflammatory response at the site of injection. but if you detect anything after 7 days it's most likely a tumor.
best of luck