What is the best strategy to evaluate the role of inflammatory cytokines in acute myocardial infraction?

More Paulo Magno Martins Dourado's questions See All

Any suggestion of free of charge questionnaires that measure mood states?

I need a free of charge and short questionnaire that measures mood states. The questionnaire will be used as in a neuropsychological evaluation study. It is well known that mood states can...

16 January 2021 8,284 4 View

Why IPNI voluntarily abbreviates some people's surnames in their standard forms?

The International Plant Name Index is known for standardising plant scientific names, including the standard forms of authors' names. I heard that one person's standard form may be chosen when...

01 January 2021 1,928 5 View

What are the largest scientific paper repository (e.g. Web of Science, Scopus) in the world? Is there a website that shows this classification?

I would like to know a hierarchy of the five largest scientific journal articles bases, for example.

21 September 2020 1,768 9 View

Have you considered publishing your research on COVID-19 in SEMINA: Exact and Technological Sciences??

I am an editor at Semina: Exact and Technological Sciences, an open, no fees journal, maintained by the State University of Londrina, Brazil. See journal information at:...

06 September 2020 3,510 14 View

Glass or PVC material to perform a soil leaching experiment of pharmaceuticals?

Hello I am developing a soil column experiment to study pharmaceuticals transport and attenuation.I would like to know If is better to use a glass or a PVC column to perform this experiment and...

10 August 2020 6,214 3 View

How do I reduce basal phosphorylation in PBMCs for phosphoFlow detection?

I'm measuring the phosphorylation status of different proteins (e.g. NFKB, ERK, AKT1, p38 etc) using phosphoflow cytometry. The protocol for surface and intracellular staining works well, all the...

22 July 2020 3,233 8 View

Hi! Someone has installed Pardiso library to work with Matlab on Linux?

I am having some problems when I try run a program with Pardiso. I seems that the Pardiso has problems with the BLAS library from Matlab. Is it correct?

28 May 2020 1,276 5 View

How to make the student participate in the virtual classroom during the COVID-19 pandemic?

We are facing a difficult problem during the pandemic: the number of students attending virtual classes has decreased significantly at the undergraduate level.

20 May 2020 5,122 27 View

How sure are you, that after TUR-B for NMIBC, to have enough muscle tissue for correct histopathological examination?

Deep resection for correct histopathological analysis in TUR-B can be challenging for the surgeon with a low to mid-experience level. This leads to inadequate analysis, possibly postponing radical...

11 April 2020 5,713 3 View

How are you guys dealing with quarantine in terms of fieldwork and laboratory analysis?

In my case, there are some studies that my team and I need to collect data monthly in situ for laboratory analysis (major ions and stable isotopes), such as rainwater, surface water, and...

24 March 2020 7,046 10 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

Ioakim Spyridopoulos

We are currently conducting a study in patients following primary PCI, where we quantify infarct size and reperfusion injury by cardiac MRI, and in parallel measure inflammation and leukocyte populations at different time points. We chose time 0 before reperfusion, 15, 30, 90 min, 24h and 3 months post infarct/reperfusion. We also looked at Th1, Th2 and Th17 response. Regarding cytokines, it is a question of money how many you can quantify and how many time points. Using Mesoscale assays from MSD for example, you can measure 30 cytokines in parallel from microliter serum. The question is however, what exactly are you interested in ? Obviously measuring some Th1 cytokines and some Th2 cytokines would give you a representative selection. TNF-alpha, IL-6 and IL-10 are regarded as very important regulators in the acute and chronic inflammation setting. We also quantify lymphocyte subsets etc.., in order to understand the dynamic better.

George Nikov Chaldakov

PAULO,

See the attached paper as a start in the field...

George

Article Adipobiology of Disease: Adipokines and Adipokine-Targeted P...

Talk continuing...

SALUT-NAZDRAVE!!!!

Article Adipoparacrinology of Atherosclerosis: Evidence Updated

Salut...

Article Adipoparacrinology: An Emerging Field in Biomedical Research

Paulo Magno Martins Dourado

Thank you Dr. George,

I will read it today.

Cheers,

Paulo

Dear PAULO, attached is 2013 issue of BMR, you are welcome TO CONTRIBUTE TO 2014 ISSUE, MS receipt before 1 November 2014, many thanks!!!

Next message will attach 2013 issue of Adipobiology, the same WELCOME...

Best of health and friendship!!!!

And please convey our Bulgarian best regards

to your President, DILMA RUSSEV, her father is Bulgarian.

Cordially yours,

George Chaldakov

Editor-in-Chief

PAULO, attached is 2013 issue of Adipobiology, WELCOME!!!!

Thank you again Dr. Chaldakov.

Rahul Dange

I suggest collecting the plasma (from blood in EDTA ) from patients with AMI, while collecting it for other lab tests. The plasma could be stored at -80 degrees and then various cytokines of interest could be estimated from it (TNF alpha, IL1Beta, AngII, IL-10 etc) using commercial ELISA kits. One late stage pro-inflammatory cytokine of interest is HMGB1. It is found to be a good indicator of the subsequent mortality post-AMI. Good luck !

Dear Colleagues,

NGF and BDNF may also be considered in coronary atherosclerosis, attached is our related paper on ACS...