What is the best strategy to evaluate the role of inflammatory cytokines in acute myocardial infraction?

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Ioakim Spyridopoulos

We are currently conducting a study in patients following primary PCI, where we quantify infarct size and reperfusion injury by cardiac MRI, and in parallel measure inflammation and leukocyte populations at different time points. We chose time 0 before reperfusion, 15, 30, 90 min, 24h and 3 months post infarct/reperfusion. We also looked at Th1, Th2 and Th17 response. Regarding cytokines, it is a question of money how many you can quantify and how many time points. Using Mesoscale assays from MSD for example, you can measure 30 cytokines in parallel from microliter serum. The question is however, what exactly are you interested in ? Obviously measuring some Th1 cytokines and some Th2 cytokines would give you a representative selection. TNF-alpha, IL-6 and IL-10 are regarded as very important regulators in the acute and chronic inflammation setting. We also quantify lymphocyte subsets etc.., in order to understand the dynamic better.

George Nikov Chaldakov

PAULO,

See the attached paper as a start in the field...

George

Article Adipobiology of Disease: Adipokines and Adipokine-Targeted P...

Talk continuing...

SALUT-NAZDRAVE!!!!

Article Adipoparacrinology of Atherosclerosis: Evidence Updated

Dear PAULO, attached is 2013 issue of BMR, you are welcome TO CONTRIBUTE TO 2014 ISSUE, MS receipt before 1 November 2014, many thanks!!!

Next message will attach 2013 issue of Adipobiology, the same WELCOME...

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Editor-in-Chief

Rahul Dange

I suggest collecting the plasma (from blood in EDTA ) from patients with AMI, while collecting it for other lab tests. The plasma could be stored at -80 degrees and then various cytokines of interest could be estimated from it (TNF alpha, IL1Beta, AngII, IL-10 etc) using commercial ELISA kits. One late stage pro-inflammatory cytokine of interest is HMGB1. It is found to be a good indicator of the subsequent mortality post-AMI. Good luck !

Dear Colleagues,

NGF and BDNF may also be considered in coronary atherosclerosis, attached is our related paper on ACS...