What is the best stem cell to regenarate myocardio tissue?

We are researching about myocardium regeneration.

01 February 2018 842 0 View

What is the optimum method to regenerate myocardium tissue?

We are researching about tissue engineering

01 February 2018 7,928 0 View

I want to know. What are the main mechanical test it is necessary to do in scaffolds obtained by collagen synthesis?

05 June 2017 405 0 View

I want to know. Is the electrospinning a process like 3D printing procedure?

We are begining research about electrospinning to obtain polymer scaffold.

01 January 1970 2,572 0 View

I want to know, which biodegradable polymer I can use to do the polymerization in printer 3D laser?

I am working in the manufacture of scaffolds with the printer 3D laser. But I do not know which is the optimum polymer. This polymer has to be biodegradable.

01 January 1970 3,108 1 View

I want to know. What is the optimum size of porous used in drug delivery system manufactured of hydrogel to coating of vascular stents?

We want to obtain surface layer of hydrogels to coat vascular stents.

01 January 1970 9,978 0 View

I want to know, which are the drug delivery system of hydrogels that can I use to coat vascular stents?

I am researching in the obtaining DDS of hydrogels to coating stents vascular.

01 January 1970 3,202 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View