What is the best stem cell to regenarate myocardio tissue?

We are researching about myocardium regeneration.

01 February 2018 842 0 View

What is the best stem cell to regeneration myocardium tissue?

We are researching about myocardium tissue engineering

01 February 2018 2,510 0 View

I want to know. What are the main mechanical test it is necessary to do in scaffolds obtained by collagen synthesis?

05 June 2017 405 0 View

I want to know. Is the electrospinning a process like 3D printing procedure?

We are begining research about electrospinning to obtain polymer scaffold.

01 January 1970 2,572 0 View

I want to know, which biodegradable polymer I can use to do the polymerization in printer 3D laser?

I am working in the manufacture of scaffolds with the printer 3D laser. But I do not know which is the optimum polymer. This polymer has to be biodegradable.

01 January 1970 3,108 1 View

I want to know. What is the optimum size of porous used in drug delivery system manufactured of hydrogel to coating of vascular stents?

We want to obtain surface layer of hydrogels to coat vascular stents.

01 January 1970 9,978 0 View

I want to know, which are the drug delivery system of hydrogels that can I use to coat vascular stents?

I am researching in the obtaining DDS of hydrogels to coating stents vascular.

01 January 1970 3,202 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View