I am searching for a spin trap for the detection of nitrogen radicals which work in photosynthetic membranes.
the larger and more insature is the molecule the more stable is the radical (you spread a charge on a wider space polar groups that attract electron in connected may help)
I think DMPO is not very specific and will do some problem with carbon -centered radicals
Everything is about ratio. So if you are expecting a descent yield of N-radicals, you can still use DMPO. You'll be able to easily distinguish between N- & C-centered radicals using EPR. Importantly, you need to make sure that during processing your samples you do not introduce cosubstrates which could be co-oxidized and give rise to secondary radicals from the anticipated N-radicals.
Can any one site one paper who demonstrated detection of nitric oxide with DMPO along with the material and methods for EPR measurements........
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