In a RT-PCR for diagnosis of blue tongue virus what is the best source of viral RNA blood or serum
For my protocol, at end-point I have to do tail-vein injections of TRITC-labeled dextran (100 ml of 10 mg/ml) and FITC-labeled lectin (50 mg). However, the protocol I'm following states that they...
01 May 2024 8,090 1 View
Hi all, In my lab we are currently differentiating neurons from hiPSCs according to the protocol used in Satir, T et al, 2020 (doi: 10.1038/s41598-020-57516-7). We have been successful in the...
27 March 2024 7,900 0 View
HemInozoin cristals are very compact in human parasites. However in opther haemoparasites as order haemosporidia is observed volutin. Do you think is there any correlation between protein...
26 February 2024 3,806 2 View
We want to conduct the sugar profile (Arabinose, Xylose, Galactose etc...) of a fermentable feedstock from different varieties. To reduce the degradation chances of this fermentable feedstock we...
10 January 2024 1,548 3 View
how fast do DNA and RNA fragments diffuse from an agarose gel?
15 August 2023 7,142 5 View
I am synthesizing carbon dots with ethanol as the solvent. I tried to do oven drying but they stick on the wall of the glass vial. I tried to do air drying also but still the same. I tried to do...
21 June 2023 3,838 4 View
Hi :) I am about to start a project in the circadian field and I will need to decide from now on which vendor I am going to select to purchase my animals. I would like to ask you guys if somebody...
29 May 2023 1,441 0 View
I am trying to neutralize nitric acid since the pH of my solution drops to zero after nitric acid treatment of my sample.
18 May 2023 6,411 3 View
Dear colleagues, consultants, and builders, I hope this message finds you well. I am reaching out to request your kind cooperation and support in disseminating the following survey. This survey is...
09 May 2023 7,674 0 View
Hi i am looking about references of posible asociation of CAPN13 gene and meat quality in cattle if you have information i be grateful
01 May 2023 2,728 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View