What is the best selective medium to isolate the etompathogenic Metarhizim anisopliae from soil samples?

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Oadi Matny

Hi: please find attached file for what you want.

Hugo Madrid

Dear colleague,

Metarhizium is quite common on plant material, too. Consider using sterilized plant material as a baiting method on your soil samples.

Success with your research!

Hugo

John E Hallsworth

Dear Mohammed, one prong of your approach could be to use the biophysical conditions that tend to favour M. anisopliae: the optimal temperaure, water activity and pH for instance (Appl Environ Microbiol 1996 62: 2435-2442; J Invert Pathol 1999 74:261-266). With regards, John

Mohammad Ibrahium Al-Masri

Dears John, Hugo, and Oadi

Thanks alot for yours answers,

Happy new year, Best Wishes

Mohammed Saleem Ali Shtayeh

Dear Mohammad,

You may like to check out the following paper:

Chandler, D., hay, D., and Reid, A.P., (1997). Sampling and occurrence of entomopathogenic fungi and nematodes in UK soils. Applied soil ecology. 5: 133-141.

You may like to see the attached paper as well.

Good luck

Attached is yet another source on the isolation of entomopathogenic fungi.

Thanks