These links are useful

http://onlinelibrary.wiley.com/doi/10.1038/oby.2005.72/pdf

Validation of Endogenous Control Genes in Human Adipose Tiss...

Regards

Dear Laura, you need to find the most stable gene in adipose tissue under your control and test conditions. Stability may vary under different conditions. Try using Genorm Application.

Hello Laura.

It may be a fashion of publishing similar kind of papers that followed one paper among the clinical people with misleading statistics.

It will pass away in very near future.

We understand how the tissues are collected and that the tissues are not entirely one of kind (a mixed origin of tissues with blood cells and body fluid, not too mention of easily breakable adipose cells, causing mRNA degradation). beta-Actin would be a credential one in terms of cell and molecular biology whatever the cell phenotypes are.

Regards.

Hi Laura Jackisch ,

In the following database (HT Atlas) you can accessed a reliable list of stably expressed housekeeping genes in adipocyte tissue and other human and mouse cell/tissue types (www.housekeeping.unicamp.br). HT Atlas also offers some primers for qPCR normalization. I hope this helps you.