Laura Jackisch

3 Questions 2 Answers 0 Followers

Questions related from Laura Jackisch

What is the best reference gene for qRT-PCR in human adipose tissue?

Hi, Does anyone have any experience with which housekeeping gene varies the least in human subcutaneous and omental adipose tissue for qRT-PCR?

22 January 2018 990 4 View

Which markers are best to prove the absence of macrophage number/activity?

Hi, I am trying to prove that my adipocyte cell line has no contamination of macrophages using rtPCR, does anyone have any experience in accepted markers of macrophage number/activity which would...

15 November 2016 9,539 3 View

Having problems extracting RNA from cells with high lipid contents, any solutions?

I'm working on primary adipocytes with a very high lipid content - to harvest the cells for RNA extraction I have used RLY lysis buffer from the Bioline RNA mini kit. However after extracting RNA...

08 March 2016 3,705 3 View