Hi, use the CTAB method or use a kit. Cheers, Nadine

Hi,

I used this protocol to extract DNA from a wide range of invertebrates:

DNeasy Blood & Tissue Kit

Because there was spin column that purified the DNA extraction.

In the case where tissue contains a lot of chitin, I used a mechanical gridding using Tungsten beads.

Hello

I have had great success extracting DNA from arthropods via the CTAB method or the DNeasy Blood and Tissue Kit, as mentioned above!

I found that I obtained higher yields from the kit, but CTAB was much cheaper!

Hello,

I found scientific information which mention PrepMan Ultra Sample Preparation Reagent for rapid DNA extraction is very useful. Also, the DNeasey B&T kit mentioned above works good.

Regards

I use a protocol from the Vienna Drosophila Resource Centre at this link:

http://stockcenter.vdrc.at/images/downloads/GoodQualityGenomicDNA.pdf

For a very effective purification of genomic and mitochondrial DNA from insects I use the attached protocol which I modified from the publication (PMID 19324101).