I attached the pics of protocol which i follow in my lab for RNA isolation from Candida and it works well with everybody in my laboratory (with the kit used is from Qiagen).  I can see you mentioned "growing on plate" you and grow them in same medium in broth and after overnight growth you can perform RNA extraction. You can refer qiagen protocol too.  Let me know if have any doubts regarding 

Hi Sharifah

You can use CTAB based method (manual protocol) or kit as well. As rightly suggested by Dhirend, the best way is to growth the cultures in liquid medium overnight (or even more, depending on species, especially filamentous fungi are slower to growth than yeasts). After that, to obtain best results, I suggest to filter dry and lyophilize the mycelia. Regarding kit, I tried both Qiagen Rneasy and the Spectrum Plant Total RNA Kit from SIGMA ALDRICH and I can assure that the Spectrum Plant Total RNA Kit can give you higher yield and higher quality.

Yes I, agree with you Fabiano.. Best wishes Sharifah. 

@Fabiano and Dhirend, Thank you so much for your suggestion.

Acid phenol method from Martine Collart. Suitable for S. cerevisiae

Collart, M.A., and Oliviero, S. (1994). Preparation of yeast RNA. In Current Protocols in Molecular Biology, F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl, eds. (New York: John Wiley & Sons), pp. 13.12.11–13.12.12.

Dear sharifah 

i hope happy day for you

 sory for late response 

i think the Total RNA Purification Maxi Kit - 8 preps form bio-world company is suitable

http://www.bio-world.com/productinfo/1_12_128/126036/Total-RNA-Purification-Maxi-Kit-preps.html

thanx