Dhirendra K. Singh

6 Questions 18 Answers 0 Followers

Questions related from Dhirendra K. Singh

Is it good to do whole genome sequencing (WGS) rather than doing16s and ITS sequencing separately?

I want to look at the bacterial and fungal diversity in some samples. I am wondering if its better to go for WGS rather than going of 16s and ITS sequencing separately? if yes, then what are the...

06 June 2022 5,027 0 View

Do anybody has idea about in silico tools which can give me idea to find protease specific cleavage sites on some specific substrates?

I have amino acid sequence of some aspartic acid proteases (not very common) from fungus. I want to check the specific restriction sites of these proteases on some known substrates using in silico...

12 October 2017 4,353 3 View

What is the role of Phorbol 12-myristate 13-acetate (PMA) in the induction of Complement Receptor 3 (CR3) or CD11b/CD18 and CR4 or CD11c/CD18?

Role of PMA on the expression and function of CR3 and CR4 expressed on innate immune cells.

28 May 2017 2,944 2 View

Can anyone please suggest me, what annealing temperature is generally suitable for designing DIG ( digoxigenin) labelled probe (probe size 400-500 bp?

I'm setting up a PCR reaction for amplifying a sequence to design Southern blot DIG labelled probe, of size 450bp. For that I set up of two reaction one is control with normal dNTP's and other is...

15 December 2016 3,609 3 View

Can I use Tango buffer instead of EcoR1 restriction buffer while digesting plasmid with EcoR1?

I performed restriction digestion of a plasmid using EcoR1 enzyme but I run out of EcoR1 buffer so I used Tango buffer (yellow) but I didnt get result. So I'm curious to know can I use tango...

26 June 2016 2,776 4 View

How can I isolate pure DNA from saliva by manual procedure?

I need to isolate pure DNA without using kit, please tell me procedure if anyone knows?

30 May 2015 1,009 9 View