Dhirendra K. Singh

11 Questions 26 Answers 0 Followers

Questions related from Dhirendra K. Singh

Is it good to do whole genome sequencing (WGS) rather than doing16s and ITS sequencing separately?

I want to look at the bacterial and fungal diversity in some samples. I am wondering if its better to go for WGS rather than going of 16s and ITS sequencing separately? if yes, then what are the...

06 June 2022 5,085 0 View

Do anyone have idea to measure Lactic acid concentration in bacterial culture media without using commercial kit?

I am using some Lactobacillus strains in my study and I want to measure the production of Lactic acid by these bacteria. I am aware of several kit (abcam, cyman, sigma) which can help me to...

25 May 2019 9,298 5 View

Do anybody has idea about in silico tools which can give me idea to find protease specific cleavage sites on some specific substrates?

I have amino acid sequence of some aspartic acid proteases (not very common) from fungus. I want to check the specific restriction sites of these proteases on some known substrates using in silico...

12 October 2017 4,380 3 View

What is the role of Phorbol 12-myristate 13-acetate (PMA) in the induction of Complement Receptor 3 (CR3) or CD11b/CD18 and CR4 or CD11c/CD18?

Role of PMA on the expression and function of CR3 and CR4 expressed on innate immune cells.

28 May 2017 2,986 2 View

I want to ask about statistics used in analyzing growth curves of the microorganism?

I have two strains A and B. I grow them in a media for 0-24 hrs and took reading at every 30 mints. I plotted these values on a graph (Y=O.D and X=Time). I want to see the significance difference...

08 February 2017 3,933 3 View

Can anyone please suggest me, what annealing temperature is generally suitable for designing DIG ( digoxigenin) labelled probe (probe size 400-500 bp?

I'm setting up a PCR reaction for amplifying a sequence to design Southern blot DIG labelled probe, of size 450bp. For that I set up of two reaction one is control with normal dNTP's and other is...

15 December 2016 3,644 3 View

Can I use Tango buffer instead of EcoR1 restriction buffer while digesting plasmid with EcoR1?

I performed restriction digestion of a plasmid using EcoR1 enzyme but I run out of EcoR1 buffer so I used Tango buffer (yellow) but I didnt get result. So I'm curious to know can I use tango...

26 June 2016 2,822 4 View

What are the main difference between electrocompetent E.coli cells and chemically competent E.coli cells?

What are the main differences between electrocompetent cell and chemically competent cells? Are they have different electroporation efficiencies?

23 June 2016 3,462 5 View

How does lithium acetate affect the cell wall integrity and why it is used during electroporation?

Specifically, my question is why we use LiAc during electroporation in yeasts?

01 March 2016 4,037 3 View

Why 1XTE is used for washing of cells during electroporation?

As I know components of 1XTE, tris act as buffer and EDTA as chelating agent. It also has a role in the protection of nucleic acid but I am wondering why it is used as wash buffer in...

01 March 2016 3,455 2 View

How can I isolate pure DNA from saliva by manual procedure?

I need to isolate pure DNA without using kit, please tell me procedure if anyone knows?

30 May 2015 1,053 9 View