What is the best protocol for NBT staining in tomato leaves for detection of superoxide radicals?

More Praveen Roylawar's questions See All

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What could be the possible reason for the drastic variation in Qmax value for same adsorbent onto two different cationic dyes itself?

The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?

30 January 2021 1,420 3 View

Where to add metamaterial unit cells in microstrip patch antenna to enhance the antenna parameters?

In order to enhance the antenna parameters, meta-material unit cells are added but where to load them is more advantageous either at ground plane or at the patch side, also is it necessary to add...

24 January 2021 2,999 4 View

How to represent Input data as multinomial distribution of feature vectors?

Hello everyone, Is there any approach to generate a fixed no. of feature vectors from the sequential data(similar to time series data...) and also express the same sequential data in terms of the...

29 November 2020 5,508 3 View

How to solve the HAZE problem in Low emissivity coatings ?

Dear friends, I am facing a problem with an issue of milky Haze in the coatings developed for aesthetic applications. All the layers were deposited by magnetron sputtering having thickness in...

08 November 2020 2,853 3 View

Lithium ion cell holder for Samsung 21700 - 50E cells?

Hello everyone, I have 5 Samsung 21700 - 50 E (NCM chemistry) cells. I want to do basic charge and discharge by connecting it in series. Does anyone know if there is a cell holder available...

05 October 2020 6,133 3 View

How the surface waves are linked to the dielectric constant of the substrate of an antenna?

How the surface waves can be reduced by choosing an appropriate substrate.

04 October 2020 8,609 3 View

What is the difference between PLSR and PLS-DA? Can we use PLSR for classification and prediction studies?

In all the textbooks related to chemometrics, PLS has been described as a regression technique that requires a dependent and independent variable for generating a regression line. However, in some...

17 August 2020 4,779 3 View

Nanotechnology in the field of nanofertilizers ?

I want to know the most important and productive role of nanotechnology in nano-fertilizers with some examples. Please add some knowledge to this topic. How much nanotechnology has grown in this...

08 August 2020 1,909 8 View

How to design double layer silver low emissive coating?

Dear friends of the research community, I am having an issue with double-layer low emissive coating fabrication. the coating of silver layer is not been adhere to the glass surface in this...

04 August 2020 7,730 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Elisa Graña

Dear Praveen,

take a look at this paper: http://link.springer.com/article/10.1007%2Fs00344-011-9250-8

In this paper you can also find how to properly remove chlorophyll.

Good luck!!

Praveen Roylawar

I tried this protocol....leaves got stained with this...but i unable remove chlorophyll..chlorophyll is partially get removed...i thank you for your positive reply...

What you need to do is remove the already 'green' ethanol for a new one, until you don't see green parts. It takes some days, but at the end, leaves turn white.

Jian Chen

After staining, you can put leaf into boiling ethanol (96%) to remove chlorophyll without damaging leaf. The detailed method is attached here. I had tried this protocol and gotten very good result.