What is the best protocol for genomic DNA extraction from tissues paraffin sections?

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What plants have high levels of sulfur-containing amino acids such as methionine?

Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...

22 July 2024 4,273 4 View

How many cells are used usually for sphere assay (E0771 cell line ) ??

I have a problem with number of cells in E0771 cell line . I usually use 100-2000 cells for sphere but I couldn't get good sphere.

04 July 2023 1,099 6 View

What is the best test to compare continuous dependent variable between 2 groups of overlapping data?

I tried to use Mann-whitney test, but when i read the assumptions it shouldn't be overlap between the samples. Should I use Wilcoxon test ? But they are not the same study samples as well Thanks

13 August 2022 2,552 5 View

Is there any feature in the Microsoft word that help you making a more professional graphical abstract?

A graphical abstract is designed to help the reader quickly gain an overview of a research article. It is therefore imperative and adds value to your paper presentation. It will be good if there...

23 July 2022 7,096 6 View

What are the most recent effective drought stress resistance mechanisms applied to vegetable plants?

Water scarcity is the universal problem both in developing and developed countries. Numerous researchers have discussed different strategies to cope with this problem. I am looking for the recent...

19 April 2022 4,442 14 View

How does Zinc interact with Iron in plants?

Is there competitive interaction between Zinc (Zn) and Iron (Fe) in plants?? Can I add both in the same solution for the foliar application?

05 January 2022 8,195 4 View

How to treat plants with nanochitosan?

Nanomaterials including nano chitosan solubility is a common problem. It could be dissolved in 1% acetic acid, however, is this suitable to treat plants with ?

19 December 2021 4,448 4 View

Hemicellulose and lignin, which are more plentiful and more degradable by microbes in lignocellulosic materials??

Cellulose, hemicellulose, and lignin are three polymers. Both cellulose and hemicellulose are polysaccharides, however, lignin is an amorphous polymer that protects the cellulosic structure from...

12 November 2021 4,535 6 View

my citations on google scholar are increasing and so my H. index..but some of them not appeared on my RG account.why? any one has a solution???

any recommendations???

23 August 2021 8,248 13 View

What is the best software for microsatellite statistical analysis ?

I have made detection for microsatellite SSR markers in patient with plasmodium... for statistical analysis, I have used genalex 6.5 ... I still need to make confirmation with another...

14 July 2021 7,887 6 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View