What is the best program of gene design using primers overlapping ?

12 December 2017 0 3K Report

I will design a synthetic gene using qualitative PCR by overlapping primers for further cloning into E. coli

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Science method

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How to calculate the annealing temperature of a primer pair?

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How to address this issue in the analysis of Real-Time PCR results?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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