What is the best primer sequence for detecting Trichomonas vaginalis infection using PCR technique?

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Unusual intensity drop in some sections of chromatograms in DDA?

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Can you suggest reliable sources defining "3D mesh" and "3D city models"?

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Absorption coefficient of methane?

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What is the best sampling strategy?

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How to confirm the site-directed mutagenesis result without performing NGS?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Why my colony PCR results of my recombinant bacterial not showing any results?

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Low-yield gel extraction problem?

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I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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Maurice Ekpenyong

Your best bet is to design your primer to target a specific component of genomic sequence of interest. Article Diagnosis of Trichomonas vaginalis Infection by PCR Using Va...

https://jcm.asm.org/content/51/4/1298

Ali Khaleghian

Thank you for sharing your research

Hala Ridha Alfahham