We use H2DCFDA to measure cellular ROS in our lab.

When properly used in the right platforms, hydroethidine (HE) and its mitochondrial version MitoSOX can give acceptable results for superoxide detection. 

Imaging is not a modality advised for these probes, which give common artifacts upon DNA binding. 

MitoSOX has been applied in FACS, and can be used in 96-well plates. (Dose range is < 2.5 uM, 15 min). 

http://www.pnas.org/content/103/41/15038.abstract

http://www.nature.com/nprot/journal/v3/n6/full/nprot.2008.56.html

Concerning limitations and problems of other ROS probes, see this thread: 

https://www.researchgate.net/post/Can_DCFH-DA_cause_cell_death_when_added_to_measure_ROS_level

here in our lab we use H2DCFDA. This method is useful for tissues and cells.

In addition you can measure the biomarkers of Oxidative Stress cell lysate such as 8-OH-dG for DNA damage, Total Hydroperoxides and Isoprostanes for lipid damage and AOPP or COgroup for protein damage. 

About DNA damages you can measure the comet assay. I don´t know if 8-OH-dg can be used as a measure of dna damages. Lipid peroxidation can be evaluated by different methods: TBARS, FOX(xilenosl orange), Dienes conjugates and probably isoportanes. For protein damages you can use the method of protein carbonilated.

Mauricio why you have doubt about 8-OH-dG? Is a well recognized marker of DNA oxidative damage. About lipid peroxidation FOX and TBARS are very old methods with many concerning. While Isoprostaes and other prostanoids such as Neuroprostanes and dihomo Isoprostanes are very reliable. About protein damage there are also some more specific markers (ie. Dityrosine). While if you think that also NOS pathway is involved you can measure Nitrotyrosine.