The optimal pH for Schiff base formation can vary depending on the specific reactants and conditions.
Generally, a mildly acidic to neutral pH (around 6-7) is suitable for the condensation reaction. Adjust the pH using a buffer solution or by controlling the acidity of the reaction medium.
Formation of Schiff base works best at neutral pH.
Alkaline conditions may result in the conversion of guanine into xanthine by hydrolysis of the 2-amino group of guanine.
Acid conditions may slow down the formation of the Schiff Base by protonation of the 2-amino group which is the driving force of the Schiff base formation.