Hi,

I am interested in cancer derived IgG especially in MCF7 (breast), PC3 (prostate) and BXPC3 (pancreatic)?

What is the best permeabilization buffer to detect the cytoplasmic IgG by flow cytometry , what is the best positive control? (A previous paper has shown IgG on cell membrane of PC3, wondering if anyone has got the same result as this paper !!

http://www.ncbi.nlm.nih.gov/pubmed/23117283