Omar Elaskalani

4 Questions 7 Answers 0 Followers

Questions related from Omar Elaskalani

Is it necessary to serum starve cancer cells for studying effect of cytokine on cell proliferation?

Hi I am working on a panel of pancreatic cancer cells studying the effects of cytokines on cell behaviour. BXPC3 especially grow well in RPMI media with 10% serum, however when I grow them on...

03 February 2016 9,045 6 View

What is the IC50 of Gemcitabine in pancreatic cancer cells?

Hi, I am working on Gemcitabine resistance in pancreatic cancer cells (ASPC1, MiaPaCa2, HPAF2, CaPaN2 and BXPC3), and I should use the IC50 of Gemcitabine in all the experiments. I found different...

24 December 2015 593 12 View

How do I induce platelets aggregation with cancer cells?

Hi, I have been trying to induce platelets aggregation using different cancer cell lines but it did not work although this is reported many times in literature. I tried the following cancer cells...

19 October 2015 725 7 View

What is the best permeabilization buffer to detect the cytoplasmic IgG by flow cytometry?

Hi, I am interested in cancer derived IgG especially in MCF7 (breast), PC3 (prostate) and BXPC3 (pancreatic)? What is the best permeabilization buffer to detect the cytoplasmic IgG by flow...

01 October 2015 5,066 2 View