I am studying Salmonella infection in mice. At the endpoint our study we harvest the liver, spleen, kidneys and small intestines' from infected mice. We want to look at Salmonella dissemination, inflammation and immune cell infiltration. I have come across many papers that look at the liver and spleen via histology.
I was wondering of the 4 organs I've harvested which one may be the best for histological analysis.
The spleen is often considered the best organ to look at Salmonella infection and dissemination in mice, as it is a primary site of bacterial replication and accumulation. The liver is also a useful organ to examine, as it is also a common site of bacterial replication and can provide information on the host immune response to the infection. Other organs that may be examined include the lymph nodes, small intestine, and colon. It's important to note that the organs that are most relevant to examine may depend on the specific strain of Salmonella and the mouse model being used.
However, based on the information you provided, the spleen and liver are likely to be the best organs to look at for histological analysis of Salmonella infection and dissemination in mice. Both the spleen and liver are primary sites of bacterial replication and accumulation, and will likely show the most pronounced signs of infection and inflammation. Additionally, these organs can provide information on the host immune response to the infection.
The small intestine and kidneys may also be informative to examine, as the small intestine is the main site of entry for Salmonella and the kidneys may also be affected by the infection.
It is important to note that the most informative organ may depend on the specific strain of Salmonella and the mouse model being used. It's recommendable to analyze all the organs that you have harvested to get a comprehensive view of the infection process, and to compare the different findings to understand the dissemination and host response.
To determine which organ is best for histo depends on what infection model one uses. If the mice are infected intraperitoneally, then liver and spleen are best. If infected orally, intestine would be a better organ.
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