1. Store the extracted protein in a specific buffer with protease inhibitors at -20 degree. When you want to run the gel, thaw the samples, add 1x laemmli buffer+ heat at 95 degree for 10 mins and load.

2. Extracted protein in buffer + protease inhibitors + laemmli buffer+ heat at 95 degree for 10 minutes and then store at -20. Whenever you want to run the sample in gel, just take out, thaw and load.

3. Extracted protein in buffer + protease inhibitors + laemmli buffer without BME and store at -20. Just before running them in gel, add BME, heat for 10 mins at 95 degree and load..?

Which is the best method among these...? Please help.