Heat stress can be measure by a variety of variables like temperature, THU or physiological parameters. But what kind of molecular marker is reliable to determine heat stress in cattle.
Members of the Hsp70 family are strongly upregulated by heat stress. Heat shock 70 kDa protein 1A (HSP70A1A), an inducible molecular chaperone that is responsible for cellular protection against heat stress, have been reported as being associated with heat tolerance.
I agree with the comments above, but I would also include serum cortisol in a time course. To be fully predictive, all these biomarkers should be accompanied with other heat stress indicators such as breathing frequency. There are some belts able to monitor respiration frequency that can be useful.