Some of our recent SEM preparation of mouse vestibular end organs showed hair cell melting. Do not know why? Is it related to perfusion or with reaction?
hello
is this hard and soft tissue ie are the hair cells sitting on bone or sitting on soft issue ,Forgive me ,I am familiar with human vestibular system but not mice and my question relates to the preparation of the specimen. are you having hard and soft tissue both scanned or just the hair cells on basement membrane ??.the reason for this question is how you prepared the tissue could have an effect on the results ,,also does your scanning machine able to examine a i n vivo samples ?
cheers
prof Galil
The normally used method for this type of sample is critical point drying (CPD). The problem with this is that you'll need an equipment to acomplish it, but there is an optional method cheaper and faster: Use Tetramethylsilane (Spectroscopy degree). Described by Ubero-Pascal et al (Microscopy Research and Technique Volume 68, Issue 5, pages 264–271, 1 December 2005), I tried and it works! Just use the conventional protocol up to 100% ethanol and then go to Tetramethylsilane, having special care to not dry the sample in any moment. Good luck!
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