We have used chloroform for cleaning and for extraction of used N hexane. We have also tried absolute ethanol. But quality and purity of oil is not good.
Ethyl acetate is a good solvent as Zamani mentioned...have you tried ether extraction (Something with a little polarity but still hydrophobic enough to be separated from the aqueous phase)?
Hi Ahmad. This is pretty easy, I did it with my plants during both of my thesis. Take the plants and put them under a microscope, here you can use a glas pipette. Sample with the tip of the glas pipette the oily trichomes - scape them carefully. Than put the pipette tip into an organic solvent (before poured into the vial) of your choice. This one you need for your GC-MS for example. With this technic I could detect the average of the secondary metabolites (terpenes and cannabinoids). Good luck, Nils :-)
The best extraction method depends on the finished product you want to obtain. As a general lab test to get good quality and purity I suggest to go for QWET extraction by using lab/food grade 200 proof ethanol (NOT Denatured). The starting material should be dry (humidity