How magnetic technology effect salinity and pH in water? How magnetic act? What is the adverse effect of this technology?

In these days there is lot of advertisement about use of magnetic to clean irrigation water but there is no scientific evidence. How magnetic effect? What is the adverse effect of this technology?...

06 July 2019 8,045 4 View

What is the best method to extract oil and CBD (cannabidiol) from leaf, flower and seed of hemp?

We have used chloroform for cleaning and for extraction of used N hexane. We have also tried absolute ethanol. But quality and purity of oil is not good. Kindly help me in this regard

04 May 2019 6,342 3 View

How to reduced pH, Conductivity, Salinity and Total Dissolved Solids in irrigation water?

There is lot of methods mention in literature to clean water but I want to know in bulk. How we can treat water from tube-wel/ turbine etc?

31 December 2018 9,870 9 View

I need information about post doc (Plant-microbe interaction)

06 July 2017 8,122 2 View

How slow grower Bradyrhizobium compete with other fast grower Rhizobium in soil environment ?

02 March 2016 3,317 4 View

No title

07 August 2015 3,914 10 View

How taxonomist divide rhizobia into different genera?

How taxonomist divide rhizobia into different genera? for example Ensifer and Rhizobium both are fast growing. Can any one help me regarding this issue?

07 August 2015 7,798 1 View

Why did rhozobia survive in hot soil conditions but not in a laboratory when the temperature was high?

I have isolated many strains of Mesorhizobium from the nodule of Chickpea (Cicer arietinum L.) growing in the desert Thal. During summer, temperatures rise up to 50 C in Thal but bacteria...

03 April 2014 8,258 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View