Demineralization of allograft bone increases the bioavailability of matrix-associated bone morphogenetic proteins (BMPs), rendering these grafts osteoinductive.  BMP content, can be obtained by demineralization protocol, it affects graft BMP levels.  BMP-7 content of

Dear Kant:

Thanks for you complete answer. Only a doubt, if I want to purificated the proteins dissociated from the bone matrix of the acid bath, first I need to calcium and phosphate precipitate and then equilibrate the acid, after this, could I use dialysis process to protein purification? however this protocol need a lot TrisHCl buffer. Is there other option?

Thanks for your help.

Best

Thanks for you complete answer.

Hi,

I have a question, is the dialysis step necessary or could you leave the guanidine in solution when you do an ELISA?

Best,

Magnus