I'm trying to efficiently dissolve torula RNA in DEPC water. I've found some protocols online that indicate I would need to extract it? Other labs say it's not neccessary, but I still can't dissolve it.
I've tried heating it and it doesn't dissolve either.
I'm trying to make the hyb solution for a zfish whole mount in situ hyb...
The exact product I'm using is:
Ribonucleic acid from torula yeast, Type VI
R6625-25G
from SIGMA
Hallo,
I use the same kind of tRNA (stock solution 50 mg/ml), and it never dissolves completely in water, so do not worry ;-) During preparation, I usually warm up the stock solution at 60-65 C (same temperature of the in situ hybridization), just to have it a little bit more homogeneous. I also vortex the stock, prior to dispensing it, to assure the same amount of material in each aliquot.
When the tRNA solution is diluted 1/100 in the hyb solution, the limited amount of tRNA solution, even if a bit lumpy, will not create any problems in the mix.
Bye, Nat
Thank you very much Natascia. That'll let me sleep better at night.
-Dani
I tried dissolving it in water, which never happened. So eventually I had a look at the bottle containing the torula RNA which had explicitly mentioned the solubility conditions on it. For the product from Sigma, the conditions would be to dissolve it in 0.1 M acetate buffer with a pH 5.0 and is soluble up to10 mg/mL. These are the conditions under which the Ribonucleic acid from Torula utilis is completely dissolved.
Hope it helps.
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