6 Questions 13 Answers 0 Followers
Questions related from Daniela Orquera
So, I'm about to start this new project and I'm wondering if there are new trending techniques to insert LoxP sites flanking an exon in the mouse genome. We use CrispR/Cas9 system in the lab, so...
06 June 2016 7,485 2 View
What are your experiences with in situ PCR vs ISH? Is the detection threshold better for in situ PCR? What kind of equipment do I need for in situ PCR? I've read a thermocycler but... what kind of...
05 May 2016 4,654 3 View
I am having difficulties with the in situs. I use embryonic mouse tissue (days 9.5 to 12.5) that have been fixed 2h to ON (depending on the tissue and what I'm trying to do with them). The issue...
04 April 2016 5,314 7 View
I'm trying to make some DIG-labelled probes work, and I am not sure where to start trying to optimize the protocol. I'm doing ISHES on e12,5 mouse embryos, fixed ON in PFA 4%. My issue is that I...
09 September 2014 9,479 6 View
I'm interested in a bioinformatics course, but I'd need one that gives scholarships because being in Argentina, it's really expensive to travel to Europe or the USA. ¿Does any of you know of any...
08 August 2014 9,373 6 View
I'm trying to efficiently dissolve torula RNA in DEPC water. I've found some protocols online that indicate I would need to extract it? Other labs say it's not neccessary, but I still can't...
06 June 2013 9,176 3 View
