Dear Dr. Sulaiman Amur Al Alawi

Chemiluminescence tests have advantages, since the 3rd or 4th generation have a greater sensitivity and specificity for the detection of antigens (such as p24) at very early stages of infection

https://www.gob.mx/cms/uploads/attachment/file/207460/lineamientos_para_la_vigilancia_por_laboratorio_VIH.pdf

sincerely

Luis del Carpio Orantes

Dear Dr. Sulaiman Amur,

I hold the same recommendation like Luis Del. Because of the sensitivity and specificity, it can detect antigens at early stages of infection

Dear Dr. Sulaiman Amur,

Currently, fourth generation assays detect both p24 antigen together with IgM and IgG against HIV-1 and HIV-2 viruses. The HIV fourth generation platforms detect p24 by using enzyme immunoassays or chemiluminescence, and detect IgM and IgG using similar configurations to those present in third generation assays. With the fourth generation assays the window period is further reduced. These assays have a sensitivity of 99.6% and a specificity of 99.8%.

In case there is a positive fourth generation assay but negative differentiation assay, nucleic acid testing for HIV-1 should be performed. This algorithm allows for detection of acute infections.

Kind regards,

Jesus Eduardo Elizondo 

Dear Dr. Sulaiman Amur;

We have to note HIV testing requires not only the p24 antigen detection but also rapidity, simplicity, a reasonable cost, and no need for special instruments. But the Chemiluminescent remains for the moment the best method compared to the one of Electroluminescence, because it offers high sensitivity, a large linear response, relative high speed for analysis, reagent stability, low cost, and low toxicity of reagents involved.

Marcellin.

THANK YOU ALL FOR YOUR BRILLIANT ANSWERS