What is the best method to carry out and monitor enzymatic sucrose hydrolysis for invert sugar production?

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Dominique Liger

Hi there,

Invertase activity on sucrose may be monitored by determining the apparition of glucose. Glucose may be monitored continuously using for instance a coupling assay with hexokinase in presence of ATP and G6Pdehydrogenase in presence of NAD+. The stoechiometric generation of NADH is followed at 340nm. The other main coupled assay to determine glucose is glucose oxydase coupled to peroxydase in presence of a chromogenic substrate.

Alaa Jabbar Abd Al-Manhel

Hi Olaoluwa , see this link and attach file I hope its useful

http://www.jbc.org/content/68/2/265.full.pdf

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200006

Greg Cote

Sucrose inversion can also be monitored by optical rotation, using a polarimeter, or by thin-layer chromatography on silica gel 60 plates, using multiple ascents in 90% acetonitrile (aq).