Tannase activity is determined by the method of Mondal and Pati11. Enzyme solution (0.1 mL) is incubated with 0.3 mL of 1.0% (w/v) tannic acid, in 0.2 M acetate buffer (pH 5.0) at 40 °C for 30 min and then the reaction is terminated at 0 °C by the addition of 2 mL BSA (1 mg/mL), which precipitates the remaining tannic acid. A control reaction is also done side by side with heat denatured enzyme. The tubes re  then centrifuged (5,000 x g, 10 min) and the precipitate is  dissolved in 2 mL of SDS – triethanolamine (1% w/v, triethanolamine) solution and the absorbency was measured at 550 nm after addition of 1 mL of FeCl3 (0.13 M) . One unit of the tannase is defined as the amount of enzyme, which is able to hydrolyse 1μ mole of ester linkage of tannic acid in 1 min at specific condition.

How do I calculate the tannase activity ? - ResearchGate. Available from: https://www.researchgate.net/post/How_do_I_calculate_the_tannase_activity [accessed Dec 13, 2016].

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I hope this helps you sir!

You can try Miller method as well as Spectrophotometric method by Sharma et al.

Please check my publication

 Thanks for all answers its helpful .

Tannase activity is determined by the method of Mondal and Pati11. Enzyme solution (0.1 mL) is incubated with 0.3 mL of 1.0% (w/v) tannic acid, in 0.2 M acetate buffer (pH 5.0) at 40 °C for 30 min and then the reaction is terminated at 0 °C by the addition of 2 mL BSA (1 mg/mL), which precipitates the remaining tannic acid. A control reaction is also done side by side with heat denatured enzyme. The tubes re then centrifuged (5,000 x g, 10 min) and the precipitate is dissolved in 2 mL of SDS – triethanolamine (1% w/v, triethanolamine) solution and the absorbency was measured at 550 nm after addition of 1 mL of FeCl3 (0.13 M) . One unit of the tannase is defined as the amount of enzyme, which is able to hydrolyse 1μ mole of ester linkage of tannic acid in 1 min at specific condition.

Ravi kU

Please take a lock at this pdf entitled

Comparative Studies on Methods of Tannase Assay

Dhruvil Brahmbhatt , H. A. Modi.