Ravinder Kumar

7 Questions 24 Answers 0 Followers

Questions related from Ravinder Kumar

Which type of steroidal saponin commonly found in plants?

i want to quantify saponin in Aloe vera genotypes using HPLC. Which steroidal saponin to be used as marker compound?? please suggest.

09 March 2017 2,307 3 View

Problem with polymerization of non denaturing polyacrylamide Gel near the well forming comb. What are the possible reasons?

I am using Sequei-gen GT (BioRad) system with well forming comb for SSR analysis. i am facing a problem, the gel is not polymerized near the comb and well not formed properly. Near the comb the...

21 April 2016 5,824 18 View

Which type of saponin is found in Aloe vera?

i want to estimate the saponin content in crude extract of aloe vera using HPLC. which standard of saponin should i use to estimate the saponin or which type of saponin is found in Aloe vera?

03 March 2016 8,308 3 View

Can you suggest selection criteria for the selection of SSR markers from the list?

Please suggest the selection criteria for the selection of SSR markers designed from the NGS data (Transcripts). Which type of markers will be useful to study genetic diversity between different...

19 November 2014 679 6 View

How do I design the SSR markers from NGS data?

We have sequenced the plant transcriptome using next generation sequencing. Now we have to Design SSR markers from this data. Please suggest how to design these markers.

18 April 2014 603 5 View

Can someone suggest the best suited DNA isolation method for many Fungal strains?

Seeking a suggestion of any DNA isolation method for different fungal strains. Some methods are working for particular strains. But I want a method which works for different strains and also best...

03 August 2013 5,539 1 View

Can someone suggest a HPLC Method for the extraction and estimation of saponin from plants?

Please suggest HPLC method for estimation of saponin. Extraction method? estimation method? HPLC column?

13 July 2013 4,075 3 View