How is AlamarBlue treatment used in 3D cell culture?

Do I need to convert 3D tumorsphere into single cell suspension before the alamarBlue treatment in assay? Or else can I use the intact tumorsphere directly for the alamarBlue treatment? Are there...

08 September 2019 7,617 4 View

Calculate lattice constant?

What is the best method to calculate lattice constant using X-ray diffraction pattern

08 September 2018 2,970 5 View

How can i determine the activity of DNA Polymerase-1 ?

The DNA polymerase gene from Bacillus licheniformis is over expressed and purified. I need to calculate the activity of that protein but that DNA polymerase is stable only upto 60 C therefore the...

06 July 2017 4,077 5 View

What is the lowest teflon vessel volume for microwave digestion for ICP-MS?

I need to buy Teflon vessels which can hold lower volumes of sample for microwave digestion methods for ICP-MS. The current vessels are suitable for only larger volumes like 10ml. Are they any...

04 May 2017 7,125 6 View

Hi, What is the reason for not getting a Ct value for all samples in real time PCR?

31 December 2016 1,907 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View